演讲 · 嘉宾
吴强

吴强

上海交通大学

上海交通大学特聘教授，博士生导师，系统生物医学教育部重点实验室常务副主任，上海交通大学比较生物医学中心主任，科技部重大科学研究计划项目首席科学家，上海市浦江学者，中美生命科学家学会终身会员，享受政府特殊津贴。生物化学与生物物理学报 (Acta Biochimica et Biophysica Sinica)，Editor；中国神经科学学会；the Chinese Society for Neuroscience，会员；The Society for Neuroscience，正式会员；The RNA Society，会员；The American Association for the Advancement of Sciences，会员；The Sigma Xi Scientific Research Society，会员；The American Society of Human Genetics，会员；The Society of Chinese Bioscientists in America，终身会员。

研究方向：

长期从事复杂基因家族的表达调控和在体功能研究，使用分子生物学和计算生物学方法，首次发现了五十多个原钙粘蛋白（protocadherin）新基因，并阐明了原钙粘蛋白基因族（protocadherin gene family）基因组结构的内在规律。发现并开拓了AT-AC内含子剪接领域，阐明了多种第一外显子调控复杂基因家族细胞和组织特异性表达的机理；发现了尿苷二磷酸葡醛酸转移酶（Ugt）基因簇具有类似于原钙粘蛋白基因簇的基因组结构；系统地分析了原钙粘蛋白和Ugt基因谱系的表达模式；发现了原钙粘蛋白和Ugt基因簇在脊椎动物进化的分子多样性的自适性选择；建立了近二十个原钙粘蛋白基因敲除小鼠品系，开发了基于跨等位基因重组的基因簇多基因打靶的新技术方法。研究论文先后发表于Science, Cell, PNAS, Molecular Cell, Nature Genetics等期刊上。

近期发表论文：
  1. Shou J., Li J., Liu Y., Wu Q. (2018) Precise and predictable CRISPR chromosomal rearrangements reveal principles of Cas9-mediated nucleotide insertion. Molecular Cell. 71, 498-509. Epub 2018 Jul 19.
  2. Fan L., Lu Y., Shen X., Shao H., Suo L., Wu Q. (2018) Alpha protocadherins and Pyk2 kinase regulate cortical neuron migration and cytoskeletal dynamics via Rac1 GTPase and WAVE complex in mice. eLife. 7. pii: e35242. doi: 10.7554/eLife.35242. Highlighted in eLife Digest.
  3. Chen W.V., Nwakeze C.L., Denny C.A., O'Keeffe S., Rieger M.A., Mountoufaris G., Kirner A., Dougherty J.D., Hen R., Wu Q., Maniatis T. (2017) Pcdhαc2 is required for axonal tiling and assembly of serotonergic circuitries in mice. Science. 356: 406-411.
  4. Huang, H. and Wu, Q. (2016). CRISPR Double Cutting through the Labyrinthine Architecture of 3D Genomes. J Genet Genomics. 43:273-288. Invited Review.
  5. Guo, Y., Q. Xu, D. Canzio, J. Shou, J. Li, D.U. Gorkin, I. Jung, H. Wu, Y. Zhai, Y. Tang, Y. Lu, Y. Wu, Z. Jia, W. Li, M.Q. Zhang, B. Ren, A.R. Krainer, T. Maniatis, Wu Q. (2015). CRISPR inversion of CTCF sites alters genome topology and enhancer/promoter function. Cell 162: 900-910.doi:10.1016/j.cell.2015.07.038. CELL Hottest Article. Research Highlighted in Cell, 162, 703–705; Nature Reviews Molecular Cell Biology 16, 578–579.
  6. Li J, Shou J，Guo Y, Tang Y, Wu Y, Jia Z, Zhai Y, Chen Z, Xu Q, Wu, Q. (2015). Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas9. J. Mol. Cell. Biol. 7: 284-298. Cover Article.
  7. Jia Z, Guo Y, Tang Y, Xu Q, Li B, Wu Q. (2014) Regulation of the protocadherin Celsr3 gene and its role in globus pallidus development and connectivity.Mol Cell Biol. 34: 3895-3910.
  8. Wang Y., Huang H., Wu Q. (2014) Characterization of the zebrafish Ugt repertoire reveals a new class of drug-metabolizing UDP glucuronosyltransferases.Mol. Pharmacol. 86: 62-75.
  9. Guo Y., Monahan K., Wu H., Varley KE., Gertz J., Li W., Myers RM., Maniatis T., Wu Q. (2012) CTCF/Cohesin-mediated DNA Looping is required for protocadherin alpha promoter choice. Proc Natl Acad Sci USA 109: 21081-21086.
  10. Suo L., Lu H., Ying G., Capecchi M.R., Wu, Q. (2012) Protocadherin clusters and cell adhesion kinase regulate dendrite complexity through Rho GTPase. J Mol Cell Biol 4: 362-376. Cover Article